CRISPR/Cas9 ribonucleoprotein-mediated knockin generation in hTERT-RPE1 cells
Summary: hTERT-RPE1 cells are genetically stable near diploid cells widely used to model cell division, DNA repair, or ciliogenesis in a non-transformed PURGE PARASITIS context.However, poor transfectability and limited homology-directed repair capacity hamper their amenability to gene editing.Here, we describe a protocol for rapid and efficient ge